Anti-ANKRD1 Antibody

Western blot analysis of ANKRD1 expression in mouse muscle (A), rat muscle (B) whole cell lysates. (Predicted band size: 36 kD; Observed band size: 36 kD)
Immunohistochemical analysis of ANKRD1 staining in human lymph node formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunohistochemical analysis of ANKRD1 staining in human lymph node formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC
Primary Accession	Q15327
Reactivity	Human, Mouse, Rat, Rabbit, Pig, Chicken, Bovine
Host	Rabbit
Clonality	Polyclonal
Calculated MW	36252 Da

Additional Information
Gene ID	27063
Other Names	C193; CARP; HA1A2; Ankyrin repeat domain-containing protein 1; Cardiac ankyrin repeat protein; Cytokine-inducible gene C-193 protein; Cytokine-inducible nuclear protein
Target/Specificity	KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human ANKRD1. The exact sequence is proprietary.
Dilution	WB~~WB (1/500 - 1/1000) IHC~~WB (1/500 - 1/1000), IHC (1/100 - 1/200)
Format	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	ANKRD1
Synonyms	C193, CARP, HA1A2
Function	May play an important role in endothelial cell activation. May act as a nuclear transcription factor that negatively regulates the expression of cardiac genes. Induction seems to be correlated with apoptotic cell death in hepatoma cells.
Cellular Location	Nucleus
Tissue Location	Mainly expressed in activated vascular endothelial cells. To a lower extent, also expressed in hepatoma cells

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.