Anti-CATSPER1 Antibody

Western blot analysis of CATSPER1 expression in Hela (A), DLD (B), A375 (C), mouse testis (D), rat testis (E) whole cell lysates. (Predicted band size: 90 kD; Observed band size: 110 kD)
Immunofluorescent analysis of CATSPER1 staining in SGC7901 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IF/IC
Primary Accession	Q8NEC5
Reactivity	Human, Mouse, Rat, Mk
Host	Rabbit
Clonality	Polyclonal
Calculated MW	90091 Da

Additional Information
Gene ID	117144
Other Names	Cation channel sperm-associated protein 1; CatSper1; hCatSper
Target/Specificity	KLH-conjugated synthetic peptide encompassing a sequence within the center region of human CATSPER1. The exact sequence is proprietary.
Dilution	WB~~WB (1/500 - 1/1000) IF/IC~~N/A
Format	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	CATSPER1
Function	Pore-forming subunit of the CatSper complex, a sperm-specific voltage-gated calcium channel that plays a central role in calcium- dependent physiological responses essential for successful fertilization, such as sperm hyperactivation, acrosome reaction and chemotaxis towards the oocyte.
Cellular Location	Cell projection, cilium, flagellum membrane; Multi-pass membrane protein {ECO:0000250\|UniProtKB:Q91ZR5}. Note=Specifically located in the principal piece of the sperm tail.
Tissue Location	Testis-specific.

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.