Anti-Apolipoprotein L1 Antibody

Western blot analysis of Apolipoprotein L1 expression in HEK293T (A), A549 (B) whole cell lysates. (Predicted band size: 43 kD; Observed band size: 44 kD)
Immunohistochemical analysis of Apolipoprotein L1 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis of Apolipoprotein L1 staining in SGC7901 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC, IF/IC
Primary Accession	O14791
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Calculated MW	43974 Da

Additional Information
Gene ID	8542
Other Names	APOL; Apolipoprotein L1; Apolipoprotein L; Apo-L; ApoL; Apolipoprotein L-I; ApoL-I
Target/Specificity	KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Apolipoprotein L1. The exact sequence is proprietary.
Dilution	WB~~WB (1/500 - 1/1000) IHC~~1:100~500 IF/IC~~N/A
Format	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	APOL1 {ECO:0000303\|PubMed:19997494}
Synonyms	APOL
Function	May play a role in lipid exchange and transport throughout the body. May participate in reverse cholesterol transport from peripheral cells to the liver. A component of trypanosome lytic factor of human serum; plays a crucial role in killing Trypanosoma brucei by forming pores in parasite lysosomal membranes and sensitizing T.brucei to oxidation-stimulated osmotic lysis (PubMed:12621437, PubMed:16020735, PubMed:19997494, PubMed:26645690, PubMed:7723792).
Cellular Location	Secreted. Note=Internalized through the endocytic pathway into the lysosomes of Trypanosoma brucei
Tissue Location	Plasma. Found on APOA-I-containing high density lipoprotein (HDL3). Expressed in pancreas, lung, prostate, liver, placenta and spleen