Anti-CD50 (Phospho-S518) Antibody

Western blot analysis of CD50 (Phospho-S518) expression in A375 (A), Jurkat (B), Myla2059 (C) whole cell lysates. (Predicted band size: 59 kD; Observed band size: 120 kD)
Immunohistochemical analysis of CD50 (Phospho-S518) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC
Primary Accession	P32942
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Calculated MW	59541 Da

Additional Information
Gene ID	3385
Other Names	Intercellular adhesion molecule 3; ICAM-3; CDw50; ICAM-R; CD50
Target/Specificity	KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S518 of human CD50 protein. The exact sequence is proprietary.
Dilution	WB~~WB (1/500 - 1/1000) IHC~~1:100~500
Format	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	ICAM3 (HGNC:5346)
Function	Cell adhesion molecule that functions as a receptor ligand of the signaling receptor ITGAL:ITGB2/LFA-1 (lymphocyte-function associated (LFA) molecule 1) ensuring apoptotic neutrophil phagocytosis by macrophages (PubMed:1448173, PubMed:1448174, PubMed:23775590, PubMed:8325327, PubMed:8459213). Is also a ligand for integrin ITGAD/ITGB2 (PubMed:8777714). Ligand of CD209 on dendritic cells (DC) through an integrin-independent mechanism that requires Ca(2+) and that forms a first contact between DC and resting T cell, facilitating the downstream DC-T cell clustering process and DC-induced proliferation of resting T Cells (PubMed:10721994).
Cellular Location	Cell membrane; Single-pass type I membrane protein Note=Localization at uropodium is mediated through interaction with MSN.
Tissue Location	Constitutively expressed only on resting leukocytes (PubMed:8325327). No expressed on resting or cytokine-activated endothelial cells (PubMed:1448174).