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EFHD1 Monoclonal Antibody(3G2)

Immunohistochemical analysis of paraffin-embedded Human-uterus tissue. 1,EFHD1 Monoclonal Antibody(3G2) was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Rat-heart tissue. 1,EFHD1 Monoclonal Antibody(3G2) was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Mouse-testis tissue. 1,EFHD1 Monoclonal Antibody(3G2) was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunofluorescence analysis of Mouse-lung tissue. 1,EFHD1 Monoclonal Antibody(3G2)(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Western blot analysis of 1) Mouse spleen tissue, 2) Rat spleen tissue, diluted at 1:3000.
IF analysis of Hela with antibody (Left) and DAPI (Right) diluted at 1:100.

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Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC-P, IF
Primary Accession	Q9BUP0
Reactivity	Human, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Calculated MW	26928 Da

Additional Information
Gene ID	80303
Other Names	EF-hand domain-containing protein D1 (EF-hand domain-containing protein 1) (Swiprosin-2)
Dilution	WB~~WB: 1:2000 IF: 1:100-200 IHC 1:50-300 IHC-P~~WB: 1:2000 IF: 1:100-200 IHC 1:50-300 IF~~WB: 1:2000 IF: 1:100-200 IHC 1:50-300
Format	PBS, pH 7.4, containing 0.09% (W/V) sodium azide as Preservative and 50% Glycerol.
Storage Conditions	-20℃

Protein Information
Name	EFHD1
Synonyms	SWS2
Function	Acts as a calcium sensor for mitochondrial flash (mitoflash) activation, an event characterized by stochastic bursts of superoxide production (PubMed:26975899). May play a role in neuronal differentiation (By similarity).
Cellular Location	Mitochondrion inner membrane {ECO:0000250\|UniProtKB:Q9D4J1}

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol

BACKGROUND

Acts as a calcium sensor for mitochondrial flash (mitoflash) activation, an event characterized by stochastic bursts of superoxide production (PubMed:26975899). May play a role in neuronal differentiation (By similarity).

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¥ 1,500.00

Cat# AP63339

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100 µl

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¥ 2,600.00

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EFHD1 Monoclonal Antibody(3G2)

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