CD75 Polyclonal Antibody

Western Blot analysis of COLO cells using CD75 Polyclonal Antibody diluted at 1：2000
Immunohistochemical analysis of paraffin-embedded Human stomach. 1, Antibody was diluted at 1:200(4°,overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).
Immunohistochemical analysis of paraffin-embedded Human stomach. 1, Antibody was diluted at 1:200(4°,overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).
Immunohistochemical analysis of paraffin-embedded Human stomach. 1, Antibody was diluted at 1:200(4°,overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC-P, IF, ICC, E
Primary Accession	P15907
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Calculated MW	46605 Da

Additional Information
Gene ID	6480
Other Names	ST6GAL1; SIAT1; Beta-galactoside alpha-2; 6-sialyltransferase 1; Alpha 2, 6-ST 1; B-cell antigen CD75; CMP-N-acetylneuraminate-beta-galactosamide-alpha-2, 6-sialyltransferase 1; ST6Gal I; ST6GalI; Sialyltransferase 1
Dilution	WB~~Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications. IHC-P~~Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications. IF~~1:50~200 ICC~~N/A E~~N/A
Format	Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.09% (W/V) sodium azide.
Storage Conditions	-20℃

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	ST6GAL1
Synonyms	SIAT1
Function	Transfers sialic acid from CMP-sialic acid to galactose- containing acceptor substrates. In B lymphocytes, generates neuraminidase-sensitive lymphocyte cell-surface differentiation antigens, such as CDw75, HB-6 and CD76 (PubMed:1730763). Sialylates complex-type N-glycans attached on the fragment crystallizable (Fc) of IgGs confering anti-inflammatory effector functions. Preferentially monosialylates the alpha(1->3) mannose antenna of Fc glycoforms with subsequent disialylation occurring at a much slower rate.
Cellular Location	Golgi apparatus, Golgi stack membrane; Single-pass type II membrane protein. Secreted {ECO:0000250\|UniProtKB:P13721}. Note=Membrane- bound form in trans cisternae of Golgi (PubMed:20378551). Secreted into the body fluid (By similarity). Colocalizes with MGAT3 and B4GALT1 in the Golgi stacks of cisternae, including the cis-part of the Golgi stacks. {ECO:0000250\|UniProtKB:P13721, ECO:0000269\|PubMed:20378551, ECO:0000269\|PubMed:29133956}

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.