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MICA Antibody (Center)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - MICA Antibody (Center) AP8626c
    Western blot analysis of MICA Antibody (Center) (Cat. #AP8626c) in MDA-MB231 cell line lysates (35ug/lane). MICA (arrow) was detected using the purified Pab.
  • 14 - MICA Antibody (Center) AP8626c
    Formalin-fixed and paraffin-embedded human hepatocarcinoma with MICA Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • 3 - MICA Antibody (Center) AP8626c
    Confocal immunofluorescent analysis of MICA Antibody (Center)(Cat#AP8626c) with MDA-MB231 cell followed by Alexa Fluor搴?488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
  • 4 - MICA Antibody (Center) AP8626c
    Flow cytometric analysis of SK-Br-3 cells using MICA Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC-P, FC, IF, E
Primary Accession Q29983
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 42915 Da
Antigen Region 68-97 aa
Additional Information
Gene ID 100507436
Other Names MHC class I polypeptide-related sequence A, MIC-A, MICA {ECO:0000312|EMBL:CAI419071}
Target/Specificity This MICA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 68-97 amino acids from the Central region of human MICA.
Dilution WB~~1:1000
IHC-P~~1:100~500
FC~~1:10~50
IF~~1:10~50
E~~Use at an assay dependent concentration.
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsMICA Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name MICA {ECO:0000312|EMBL:CAI41907.1}
Function Widely expressed membrane-bound protein which acts as a ligand to stimulate an activating receptor KLRK1/NKG2D, expressed on the surface of essentially all human natural killer (NK), gammadelta T and CD8 alphabeta T-cells (PubMed:11491531, PubMed:11777960). Up- regulated in stressed conditions, such as viral and bacterial infections or DNA damage response, serves as signal of cellular stress, and engagement of KLRK1/NKG2D by MICA triggers NK-cells resulting in a range of immune effector functions, such as cytotoxicity and cytokine production (PubMed:10426993).
Cellular Location Cell membrane; Single-pass type I membrane protein. Cytoplasm Note=Expressed on the cell surface in gastric epithelium, endothelial cells and fibroblasts and in the cytoplasm in keratinocytes and monocytes. Infection with human adenovirus 5 suppresses cell surface expression due to the adenoviral E3-19K protein which causes retention in the endoplasmic reticulum.
Tissue Location Widely expressed with the exception of the central nervous system where it is absent. Expressed predominantly in gastric epithelium and also in monocytes, keratinocytes, endothelial cells, fibroblasts and in the outer layer of Hassal's corpuscles within the medulla of normal thymus. In skin, expressed mainly in the keratin layers, basal cells, ducts and follicles. Also expressed in many, but not all, epithelial tumors of lung, breast, kidney, ovary, prostate and colon. In thyomas, overexpressed in cortical and medullar epithelial cells. Tumors expressing MICA display increased levels of gamma delta T-cells.
Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

BACKGROUND

MICA is the higly polymorphic MHC (HLA) class I chain-related gene A. The protein product is expressed on the cell surface, although unlike canonical class I molecules does not seem to associate with beta-2-microglobulin. It is thought that MICA functions as a stress-induced antigen that is broadly recognized by intestinal epithelial gamma delta T cells.

REFERENCES

Bahram,S., et.al., Proc. Natl. Acad. Sci. U.S.A. 91 (14), 6259-6263 (1994) Klein,J.et.al., Proc. Natl. Acad. Sci. U.S.A. 91 (14), 6251-6252 (1994) Parham,P., et.al., J. Immunol. 142 (11), 3937-3950 (1989)

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