癌症的基本特征包括细胞增殖、血管生成、迁移、凋亡逃避机制和细胞永生等。找到癌症发生过程中这些通路的关键标记物和对应的抗体用于检测至关重要。
为您推荐一个泛素化位点预测神器——泛素化分析工具，可以为您的蛋白的泛素化位点作出预测和评分。
细胞自噬受体图形绘图工具为你的蛋白的细胞受体结合位点作出预测和评分，识别结合到自噬通路中的蛋白是非常重要的，便于让我们理解自噬在正常生理、病理过程中的作用，如发育、细胞分化、神经退化性疾病、压力条件下、感染和癌症。
PRUNE Antibody (C-term) 精选
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- 产品详情
- 实验流程
- 背景知识
Application 
| WB, IHC-P, FC, E |
|---|---|
| Primary Accession | Q86TP1 |
| Reactivity | Human |
| Predicted | Bovine, Rabbit |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 50200 Da |
| Antigen Region | 360-389 aa |
| Gene ID | 58497 |
|---|---|
| Other Names | Protein prune homolog, hPrune, Drosophila-related expressed sequence 17, DRES-17, DRES17, HTcD37, PRUNE |
| Target/Specificity | This PRUNE antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 360-389 amino acids from the C-terminal region of human PRUNE. |
| Dilution | WB~~1:1000 IHC-P~~1:100~500 FC~~1:10~50 E~~Use at an assay dependent concentration. |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | PRUNE Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
For Research Use Only. Not For Use In Diagnostic Procedures.
| Name | PRUNE1 (HGNC:13420) |
|---|---|
| Synonyms | PRUNE |
| Function | Exopolyphosphatase that catalyzes the hydrolysis of inorganic polyphosphates, with preference for short-chain substrates, as well as nucleoside 5'-tetraphosphates, releasing terminal phosphate residues (PubMed:18700747). Also exhibits phosphodiesterase activity toward cyclic nucleotides, hydrolyzing cAMP and cGMP to their corresponding 5'-monophosphates (PubMed:14998490, PubMed:17655525). Requires divalent metal ions for activity (PubMed:14998490, PubMed:18700747). Plays a role in cell proliferation, migration and differentiation, and acts as a negative regulator of NME1 (PubMed:10602478, PubMed:11687967, PubMed:14998490, PubMed:16428445, PubMed:17906697). Plays a role in the regulation of neurogenesis. Involved in the regulation of microtubule polymerization (PubMed:28334956). |
| Cellular Location | Cytoplasm. Nucleus. Cell junction, focal adhesion. Note=In some transfected cells a nuclear staining is also observed |
| Tissue Location | Ubiquitously expressed. Seems to be overexpressed in aggressive sarcoma subtypes, such as leiomyosarcomas and malignant fibrous histiocytomas (MFH) as well as in the less malignant liposarcomas. |
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
Phosphodiesterase (PDE) that has higher activity toward cAMP than cGMP, as substrate. It plays a role in cell proliferation, is able to induce cell motility and acts as a negative regulator of NME1.
REFERENCES
Vieira, A.R., et al. Genet. Med. 10(9):668-674(2008)
Middelhaufe, S., et al. Biochem. J. 407(2):199-205(2007)
Kobayashi, T., et al. Mol. Cell. Biol. 26(3):898-911(2006)
Zollo, M., et al. Clin. Cancer Res. 11(1):199-205(2005)
Forus, A., et al. Oncogene 20(47):6881-6890(2001)
Reymond, A., et al. Oncogene 18(51):7244-7252(1999)
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