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Anti-TROP2 Reference Antibody (sacituzumab)

Recombinant Antibody

Anti-TROP2 Reference Antibody (sacituzumab) on SDS-PAGE under reducing (R) condition. The purity of the protein is greater than 95%.
The purity of Anti-TROP2 Reference Antibody (sacituzumab) is 96.23%, determined by SEC-HPLC.
Immobilized human TROP2 His at 2 ug/mL can bind Anti-TROP2 Reference Antibody (sacituzumab), EC50=0.004316 ug/mL.
Human TROP2 HEK293 cells were stained with Anti-TROP2 Reference Antibody (sacituzumab) and negative control protein respectively, washed and then followed by PE and analyzed with FACS, EC50=0.4071 ug/mL.
The endocytosis ratio sacituzumab by human TROP2 HEK293 increased with the increase of antibody concentration, and the Internalization Rate (%) reached 70% at antibody concentration of 0.4 nM.

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Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	Functional assay, Kinetics (SPR), Kinetics (BLI), FACS, E, FTA
Primary Accession	P09758
Reactivity	Human, Mouse
Clonality	Monoclonal
Isotype	IgG1
Calculated MW	35709 Da

Additional Information
Target/Specificity	TROP2
Endotoxin	< 0.001EU/ µg,determined by LAL method.
Conjugation	Unconjugated
Expression system	CHO
Format	Purified monoclonal antibody supplied in PBS, pH6.0, without preservative.This antibody is purified through a protein A column.

For Research Use Only. Not For Use In Diagnostic Procedures.

Patent Notice: The variable region sequence of this antibody is derived from a published third-party patent. This product is manufactured independently as a Research Use Only (RUO) reagent and is not licensed, sponsored, or endorsed by the patent holder. It is intended solely for research purposes. Customers considering any commercial, diagnostic, or therapeutic use should independently evaluate freedom-to-operate with respect to relevant third-party patents.

Protein Information
Name	TACSTD2
Synonyms	GA733-1, M1S1, TROP2
Function	May function as a growth factor receptor.
Cellular Location	Membrane; Single-pass type I membrane protein.
Tissue Location	Placenta, pancreatic carcinoma cell lines.

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol