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OTUD4 Antibody

     
  • 1 - OTUD4 Antibody ASC10867
    Western blot analysis of OTUD4 in Daudi cell lysate with OTUD4 antibody at (A) 0.25 and (B) 0.5 µg/mL.
  • 8 - OTUD4 Antibody ASC10867
    Immunocytochemistry of OTUD4 in Daudi cells with OTUD4 antibody at 2.5 µg/mL.
  • 3 - OTUD4 Antibody ASC10867
    Immunofluorescence of OTUD4 in Daudi cells with OTUD4 antibody at 20 µg/mL.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IF, ICC, E
Primary Accession Q01804
Other Accession NP_001096123, 156630992
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype IgG
Calculated MW 124045 Da
Concentration (mg/ml) 1 mg/mL
Conjugate Unconjugated
Application Notes OTUD4 antibody can be used for detection of OTUD4 by Western blot at 0.25 - 0.5 µg/mL. Antibody can also be used for immunocytochemistry starting at 2.5 µg/mL. For immunofluorescence start at 20 µg/mL.
Additional Information
Gene ID 54726
Other Names OTU domain-containing protein 4, 3.4.19.12, HIV-1-induced protein HIN-1, OTUD4, HIN1, KIAA1046
Target/Specificity OTUD4; Multiple isoforms of OTUD4 are known to exist. OTUD4 antibody is predicted to not cross-react with other members of the OTUD family.
Reconstitution & Storage OTUD4 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
PrecautionsOTUD4 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name OTUD4 (HGNC:24949)
Function Deubiquitinase which hydrolyzes the isopeptide bond between the ubiquitin C-terminus and the lysine epsilon-amino group of the target protein (PubMed:23827681, PubMed:25944111, PubMed:29395066). May negatively regulate inflammatory and pathogen recognition signaling in innate immune response. Upon phosphorylation at Ser-202 and Ser-204 residues, via IL-1 receptor and Toll-like receptor signaling pathway, specifically deubiquitinates 'Lys-63'-polyubiquitinated MYD88 adapter protein triggering down-regulation of NF-kappa-B-dependent transcription of inflammatory mediators (PubMed:29395066). Independently of the catalytic activity, acts as a scaffold for alternative deubiquitinases to assemble specific deubiquitinase- substrate complexes. Associates with USP7 and USP9X deubiquitinases to stabilize alkylation repair enzyme ALKBH3, thereby promoting the repair of alkylated DNA lesions (PubMed:25944111).
Cellular Location Cytoplasm. Nucleus. Note=Primarily cytoplasmic
Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

BACKGROUND

OTUD4 Antibody: OTUD4, also known as HIV-1 induced protein HIN-1, is a member of the OTU (ovarian tumor) domain containing cysteine protease superfamily, in which the OUT domain generally confers deubiquitinase activity. At least three isoforms of OTUD4 are known to exist, and the smallest of these isoforms are only expressed in HIV-1-infected cells (provided by RefSeq).

REFERENCES

Borodovsky A, Ovaa H, Kolli N, et al. Chemistry-based functional genomics reveals novel members of the deubiquitinating enzyme family. Chem. Biol. 2002; 10:1149-59.


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