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CYK18 Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - CYK18 Antibody (C-term) AX10002
    All lanes: Anti-CYK18 Antibody (C-term) at 1:2000 dilution Lane 1: K562 whole cell lysate Lane 2: Mouse uterus lysate Lane 3: Mouse colon lysate Lysates/proteins at 20 µg per lane. Secondary: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ASP1615) at 1/15000 dilution. Observed band size: 48 KDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 14 - CYK18 Antibody (C-term) AX10002
    CYK18 Antibody (C-term)(Cat. #AX10002)immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of CYK18 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
  • 4 - CYK18 Antibody (C-term) AX10002
    CYK18 Antibody (C-term)(Cat. #AX10002) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
  • 3 - CYK18 Antibody (C-term) AX10002
    Confocal immunofluorescent analysis of CYK18 Antibody (C-term) (Cat. #AX10002)with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC-P, FC, IF, E
Primary Accession P05783
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Clone Names RB18746
Calculated MW 48058 Da
Antigen Region 401-430 aa
Additional Information
Gene ID 3875
Other Names Keratin, type I cytoskeletal 18, Cell proliferation-inducing gene 46 protein, Cytokeratin-18, CK-18, Keratin-18, K18, KRT18, CYK18
Target/Specificity This CYK18 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 401-430 amino acids from the C-terminal region of human CYK18.
Dilution WB~~1:2000
IHC-P~~1:1000
FC~~1:50
IF~~1:200
E~~Use at an assay dependent concentration.
Format Purified polyclonal antibody supplied in PBS with 0.05% (V/V) Proclin 300. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCYK18 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name KRT18
Synonyms CYK18
Function Involved in the uptake of thrombin-antithrombin complexes by hepatic cells (By similarity). When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection.
Cellular Location Nucleus matrix {ECO:0000250|UniProtKB:Q5BJY9}. Cytoplasm, perinuclear region. Nucleus, nucleolus. Cytoplasm {ECO:0000250|UniProtKB:Q5BJY9}
Tissue Location Expressed in colon, placenta, liver and very weakly in exocervix. Increased expression observed in lymph nodes of breast carcinoma.
Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

BACKGROUND

KRT18 is the type I intermediate filament chain keratin 18. Keratin 18, together with its filament partner keratin 8, are perhaps the most commonly found members of the intermediate filament family. They are expressed in single layer epithelial tissues of the body. Mutations in its gene have been linked to cryptogenic cirrhosis.

REFERENCES

Zhang,Q., Clin. Cancer Res. 15 (10), 3557-3567 (2009) Kruse,R., Folia Histochem. Cytobiol. 47 (1), 127-130 (2009) Toivola,D.M., Hepatology 40 (2), 459-466 (2004)

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