CK18 antibody

Mouse Monoclonal Antibody (Mab)

Immunohistochemical analysis of formalin-fixed and paraffin-embedded human lung adenocarcinoma tissue was performed with CK18 primary antibody, followed by peroxidase-conjugated secondary antibody and DAB staining.
Immunohistochemical analysis of formalin-fixed and paraffin-embedded human benign prostatic hyperplasia tissue was performed with CK18 primary antibody, followed by peroxidase-conjugated secondary antibody and DAB staining.
Immunohistochemical analysis of formalin-fixed and paraffin-embedded human prostatic adenocarcinoma tissue was performed with CK18 primary antibody, followed by peroxidase-conjugated secondary antibody and DAB staining.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	IHC
Primary Accession	P05783
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Clone Names	261O3V2
Calculated MW	48058 Da

Additional Information
Gene ID	3875
Other Names	Keratin, type I cytoskeletal 18, Cell proliferation-inducing gene 46 protein, Cytokeratin-18, CK-18, Keratin-18, K18, KRT18, CYK18
Dilution	IHC~~1:100~500
Storage	Maintain refrigerated at 2-8°C.

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	KRT18 (HGNC:6430)
Synonyms	CYK18
Function	Structural component of intermediate filaments in simple epithelial cells. Forms obligate heteropolymers with a type II keratin KRT8, mechanical stability and resilience to epithelial cells (PubMed:15529338, PubMed:26823019). KRT8/KRT18 filaments are involved in ARHGEF40-mediated actin stress fiber formation and tensional force- induced stress fiber formation and reinforcement (PubMed:26823019). Organization and orientation of KRT8/KRT18 filaments are responsible for the properly elongated morphology of epithelial tubules (By similarity). Also acts downstream of ROCK kinase activation as part of a positive feedback mechanism in response to cellular mechanical stress loading (PubMed:26823019). Involved in the uptake of thrombin- antithrombin complexes by hepatic cells (By similarity). When phosphorylated, plays a role in filament reorganization (PubMed:7523419, PubMed:8522591, PubMed:9298992, PubMed:9524113). Involved in the delivery of mutated CFTR to the plasma membrane (PubMed:15529338, PubMed:16424149). Involved in interleukin-6 (IL-6)- mediated barrier protection (PubMed:17213200).
Cellular Location	Nucleus matrix {ECO:0000250\|UniProtKB:Q5BJY9}. Cytoplasm, perinuclear region. Nucleus, nucleolus. Cytoplasm {ECO:0000250\|UniProtKB:Q5BJY9}
Tissue Location	Expressed in colon, placenta, liver and very weakly in exocervix. Increased expression observed in lymph nodes of breast carcinoma.