Anti-CRK (Phospho-Y221) Antibody

Western blot analysis of CRK (Phospho-Y221) expression in Hela (A), THP1 (B), mouse liver (C), rat liver (D) whole cell lysates. (Predicted band size: 33 kD; Observed band size: 46 kD)
Immunofluorescent analysis of CRK (Phospho-Y221) staining in HuvEc cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with Alexa Fluor 647-conjugated secondary antibody (red) in PBS at room temperature in the dark.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IF/IC
Primary Accession	P46108
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Calculated MW	33831 Da

Additional Information
Gene ID	1398
Other Names	Adapter molecule crk; Proto-oncogene c-Crk; p38
Target/Specificity	KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding Y221 of human CRK protein. The exact sequence is proprietary.
Dilution	WB~~WB (1/500 - 1/1000) IF/IC~~N/A
Format	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	CRK
Function	Involved in cell branching and adhesion mediated by BCAR1- CRK-RAPGEF1 signaling and activation of RAP1.
Cellular Location	Cytoplasm. Cell membrane. Note=Translocated to the plasma membrane upon cell adhesion.

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.