Phospholipase C beta 3 Rabbit mAb

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC-P
Primary Accession	Q01970
Reactivity	Human
Host	Rabbit
Clonality	Monoclonal Antibody
Isotype	IgG
Conjugate	Unconjugated
Immunogen	A synthesized peptide derived from human PLCB3
Purification	Affinity Purified
Calculated MW	138799 Da

Additional Information
Gene ID	5331
Other Names	PLCB3
Dilution	WB~~1/500-1/1000 IHC-P~~1:50~200
Format	Liquid in 10mM PBS, pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	PLCB3 {ECO:0000303\|PubMed:20966218, ECO:0000312\|EMBL:AAA77683.1}
Function	Catalyzes the production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) (PubMed:20966218, PubMed:29122926, PubMed:37172014, PubMed:37991948, PubMed:9188725). Key transducer of G protein-coupled receptor signaling: activated by G(q)/G(11) G alpha proteins downstream of G protein-coupled receptors activation (PubMed:20966218, PubMed:37991948). In neutrophils, participates in a phospholipase C- activating N-formyl peptide-activated GPCR (G protein-coupled receptor) signaling pathway by promoting RASGRP4 activation by DAG, to promote neutrophil functional responses (By similarity). In conjunction with OSBPL2, it may contribute to the control of keratinocyte proliferation and differentiation, probably through the regulation of the ERK pathway and the cell cycle (PubMed:38701954).
Cellular Location	Cytoplasm. Membrane; Peripheral membrane protein; Cytoplasmic side. Nucleus {ECO:0000250\|UniProtKB:P51432} Note=An heterotetramer composed of GNG2 and GNB1 increases the concentration of PLCB3 on the membrane surface, where its substrate PIP2 resides, by orienting its catalytic site