癌症的基本特征包括细胞增殖、血管生成、迁移、凋亡逃避机制和细胞永生等。找到癌症发生过程中这些通路的关键标记物和对应的抗体用于检测至关重要。
为您推荐一个泛素化位点预测神器——泛素化分析工具，可以为您的蛋白的泛素化位点作出预测和评分。
细胞自噬受体图形绘图工具为你的蛋白的细胞受体结合位点作出预测和评分，识别结合到自噬通路中的蛋白是非常重要的，便于让我们理解自噬在正常生理、病理过程中的作用，如发育、细胞分化、神经退化性疾病、压力条件下、感染和癌症。
CYK18 Antibody (C-term)
Mouse Monoclonal Antibody (Mab)
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Application 
| IF, WB |
|---|---|
| Primary Accession | P05783 |
| Reactivity | Human |
| Host | Mouse |
| Clonality | Monoclonal |
| Calculated MW | 48058 Da |
| Isotype | IgG3 |
| Antigen Source | HUMAN |
| Gene ID | 3875 |
|---|---|
| Antigen Region | 400-430 aa |
| Other Names | Keratin, type I cytoskeletal 18, Cell proliferation-inducing gene 46 protein, Cytokeratin-18, CK-18, Keratin-18, K18, KRT18, CYK18 |
| Dilution | IF~~1:25 WB~~1:2000 |
| Target/Specificity | This CYK18 Monoclonal antibody is generated from mouses immunized with a KLH conjugated synthetic peptide selected from the 400-430 region of human CYK18. |
| Format | Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | CYK18 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
For Research Use Only. Not For Use In Diagnostic Procedures.
| Name | KRT18 (HGNC:6430) |
|---|---|
| Synonyms | CYK18 |
| Function | Structural component of intermediate filaments in simple epithelial cells. Forms obligate heteropolymers with a type II keratin KRT8, mechanical stability and resilience to epithelial cells (PubMed:15529338, PubMed:26823019). KRT8/KRT18 filaments are involved in ARHGEF40-mediated actin stress fiber formation and tensional force- induced stress fiber formation and reinforcement (PubMed:26823019). Organization and orientation of KRT8/KRT18 filaments are responsible for the properly elongated morphology of epithelial tubules (By similarity). Also acts downstream of ROCK kinase activation as part of a positive feedback mechanism in response to cellular mechanical stress loading (PubMed:26823019). Involved in the uptake of thrombin- antithrombin complexes by hepatic cells (By similarity). When phosphorylated, plays a role in filament reorganization (PubMed:7523419, PubMed:8522591, PubMed:9298992, PubMed:9524113). Involved in the delivery of mutated CFTR to the plasma membrane (PubMed:15529338, PubMed:16424149). Involved in interleukin-6 (IL-6)- mediated barrier protection (PubMed:17213200). |
| Cellular Location | Nucleus matrix {ECO:0000250|UniProtKB:Q5BJY9}. Cytoplasm, perinuclear region. Nucleus, nucleolus. Cytoplasm {ECO:0000250|UniProtKB:Q5BJY9} |
| Tissue Location | Expressed in colon, placenta, liver and very weakly in exocervix. Increased expression observed in lymph nodes of breast carcinoma. |
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
Involved in the uptake of thrombin-antithrombin complexes by hepatic cells (By similarity). When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection.
REFERENCES
Oshima R.G., et al. Differentiation 33:61-68(1986).
Kim J.W., et al. Submitted (SEP-2004) to the EMBL/GenBank/DDBJ databases.
Kalnine N., et al. Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases.
Suzuki Y., et al. Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases.
Kulesh D.A., et al. Mol. Cell. Biol. 8:1540-1550(1988).
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